Development and validation of a lateral flow immunoassay for rapid detection of VanA-producing enterococci
نویسندگان
چکیده
منابع مشابه
Development and Validation of a Lateral Flow Immunoassay for Rapid Detection of NDM-Producing Enterobacteriaceae
The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. The NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flow immunoassay (LFIA) (called the NDM LFIA) for the rapid and reliable detectio...
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We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detection limit of the kit was 6.3 × 10(6) CFU per test. Of 127 vancomycin-resistant m...
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Allergies to cow’s milk are very common and can present as life-threatening anaphylaxis. Consequently, food labeling legislation mandates that foods containing milk residues, including casein and/or b-lactoglobulin, provide an indication of such on the product label. Because contamination with either component independent of the other can occur during food manufacturing, effective allergen mana...
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Tropicamide is an antimuscarinic agent used as eye drops for refractive examinations. The aim of this study was to develop a simple and suitable analytical method for determination of tropicamide in eye drops in the presence of excipients. A zero-crossing third and fourth derivative spectrophotometric method was described for determination of tropicamide in eye drops. The measurements wer...
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Early and accurate diagnosis of invasive candidiasis (IC) is very important. In this study, a lateral flow immunoassay (LFIA) was developed to detect antibody against Candida albicans enolase (Eno). Colloidal gold particle labeled mouse anti human IgG (1.0 mg/L) was used as the detector reagent. Recombinant enolase (rEno, 1.0 mg/L) and goat anti IgG (1.0 mg/L) were immobilized in test and contr...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 2020
ISSN: 0305-7453,1460-2091
DOI: 10.1093/jac/dkaa413